《植物生理学报》 2015, 51(12): 2263-2269

TSA对花生响应干旱及相关基因表达的影响

李丽梅, 钟钰婷, 苏良辰, 刘帅, 邓斌, 李玲^*

广东省植物发育生物工程重点实验室, 华南师范大学生命科学学院, 广州510631

通信作者：李；E-mail: liling@scnu.edu.cn；Tel: 020-85211378

摘　要：

研究组蛋白去乙酰化酶抑制剂曲古抑菌素A (trichostatin A, TSA)处理四叶期花生后对花生抗旱性和相关基因表达的影响。结果发现, 用1 μmol•L^-1的TSA处理的花生, 在干旱胁迫下, 叶片水分含量保持较高, 加速气孔关闭, 促进叶片AhP5CS表达, 增加叶片脯氨酸含量; 经TSA处理的花生在干旱处理下, 体内AhHDA1表达提高, 促进AhNCED1、AhAREB1和AhNAC2迅速表达。表明TSA通过影响组蛋白乙酰化水平参与花生幼苗对干旱胁迫的响应, 结果为认识组蛋白乙酰化影响花生的抗旱提供依据。

关键词：TSA; 花生; 干旱胁迫; 脯氨酸含量

收稿：2015-11-02   修定：2015-11-24

资助：国家自然科学基金(31471422)。

Effect of TSA on Responses and Related Genes Expression of Peanut under Drought Stress

LI Li-Mei, ZHONG Yu-Ting, SU Liang-Chen, LIU Shuai, DENG Bin, LI Ling^*

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China

Corresponding author: LI Ling; E-mail: liling@scnu.edu.cn; Tel: 020-85211378

Abstract:

This paper reveals the influences of trichostatin A (TSA), the histone deacetylase inhibitor, on drought resistance and related genes expression of peanut. The results showed that under drought stress, stomata closed in leaf of peanut treated with 1 μmol•L^-1 TSA to keep water content higher than the contrast and Ah-P5CS is up-regulated so that the proline contents of leaf increased. Furthermore, the expression level of AhHDA1 increased to promote AhNCED1, AhAREB1 and AhNAC2 expressed rapidly. This study indicates that histone acetylation influences the response of peanut seedling to drought stress, providing the basis for a further understanding of histone acetylation on drought mechanism of peanut.

Key words: TSA; peanut; drought stress; proline content